Description
Designed by cell sorting experts for today’s most challenging cell sorting applications, the Bigfoot Spectral Cell Sorter combines performance, safety, and ease-of-use to fit seamlessly into the workflow of both individual labs and core facilities. Bigfoot allows spectral unmixing or conventional compensation for high-parameter, high-throughput cell sorting experiments for maximum flexibility.
Features and Benefits
The Bigfoot Spectral Cell Sorter supports configurations with up to 9 lasers (349nm, 405nm, 445nm, 488nm, 532nm, 561nm, 594nm, 640nm, 785nm) and 60 detectors, delivering versatility for both standard fluorescence detection and spectral unmixing. Multiple scatter options enhance flexibility with simultaneous standard and small particle detection, multi-laser scatter detection, and polarization. Capable of 6-way sorting into tubes, 4-way sorting into 96 well plates and 384 well plates, or straight down sorting into 1536 well plates. A massively parallel, pipelined architecture eliminates hard aborts and allows complex, high color experiments with up to a 60 x 60 compensation matrix or spectral unmixing without limiting instrument performance. Advanced automation and software allow operators with all levels of knowledge to use and control the system with confidence. Integrated control systems ensure optimal droplet formation persistence and position stabilization over time ensuring accurate drop delay maintenance through the day. To streamline workflow and reduce user errors, automated stream calibration and drop delay simplify sort setup while bubble detection notifies the operator and stops the sort to preserve precious samples. Integrated temperature control (4-37°C) and agitation maintain sample integrity for sample input while temperature control of the output maintains the viability of sorted samples. Flexibility to support a range of cell types and applications. The Bigfoot Spectral Cell Sorter’s jet-in-air sensing is gentle with fragile cells to maximize viability at high sort speeds, minimizing impact on yield. A swap tip wizard simplifies setup of 50, 70, 100, 120, and 150 µm nozzle tips. Integrated biosafety cabinet for operator and sample protection.